pRLP121 vector (V003697)

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LOCUS       V003697                 7078 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003697
VERSION     V003697
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Hsu TM, Welner DH, Russ ZN, Cervantes B, Prathuri RL, Adams PD,
            Dueber JE.
  TITLE     Employing a biochemical protecting group for a sustainable indigo
            dyeing strategy
  JOURNAL   Nat. Chem. Biol. (2018) In press
   PUBMED   29309053
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Hsu TM, Welner DH, Russ ZN, Cervantes B, Prathuri RL, Adams PD,
            Dueber JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2017) Bioengineering, UC Berkeley, 2151 Berkeley
            Way Room 512D, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 7078)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7078)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-AUG-2017) Bioengineering, UC Berkeley, 2151 Berkeley Way Room
            512D, Berkeley, CA 94704, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(168..310)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(415..603)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(1881..1902)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1918..2997)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(2998..3075)
                     /label="lacI promoter"
     promoter        3384..3402
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3403..3427
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3442..3464
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3477..3494
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3516..3536
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             3540..4889
                     /gene="bglA"
                     /label="Beta-glucosidase"
                     /note="Beta-glucosidase from Niallia circulans. Accession#:
                     Q03506"
     terminator      4973..5020
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(5195..5223)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        5336..5440
                     /label="AmpR promoter"
     CDS             5441..6298
                     /label="AmpR"
                     /note="beta-lactamase"
     regulatory      6299..6395
                     /regulatory_class="terminator"
     rep_origin      6472..7060
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"