pRL1342 vector (V003714)

LOCUS       V003714                 8416 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003714
VERSION     V003714
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Wolk CP.
  TITLE     pRL1342, an RSF1010-derived vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Wolk CP.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2001) MSU-DOE Plant Research Laboratory, Michigan
            State University, Wilson Road, E. Lansing, MI 48824-1312, USA
REFERENCE   3  (bases 1 to 8416)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUL-2001) MSU-DOE Plant Research Laboratory, Michigan State
            University, Wilson Road, E. Lansing, MI 48824-1312, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(155..549)
                     /direction=LEFT
                     /label="RSF1010 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     RSF1010; requires the RSF1010 RepA/B/C proteins for
                     replication (Scholz et al., 1989)"
     oriT            891..978
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
     CDS             2217..3185
                     /label="RSF1010 RepB"
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             3699..4535
                     /label="RSF1010 RepA"
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             4525..5373
                     /label="RSF1010 RepC"
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     regulatory      complement(5709..5735)
                     /note="trpA terminator from Lorist6 (GenBank Accession
                     Number X98450)"
                     /regulatory_class="terminator"
     misc_feature    5758..6157
                     /label="cos"
                     /note="lambda cos site; allows packaging into phage lambda
                     particles"
     protein_bind    complement(6168..6201)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6217..6235
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    6239..6398
                     /label="polylinker (multiple cloning site)"
                     /note="polylinker (multiple cloning site)"
     regulatory      6398..6415
                     /note="SP6 promoter from pBAC108L (GenBank Accession Number
                     U51114)"
                     /regulatory_class="promoter"
     promoter        complement(6398..6415)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      6431..6457
                     /note="cat promoter from pBR325 (GenBank Accession Number
                     L08855)"
                     /regulatory_class="promoter"
     CDS             6524..7180
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             7437..8168
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     terminator      8361..8404
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     regulatory      8367..8395
                     /note="rrnC terminator from Lorist6 (GenBank Accession
                     Number X98450)"
                     /regulatory_class="terminator"