pRISE vector (V003733)

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LOCUS       40924_37088       12502 bp DNA     circular SYN 18-DEC-2018
DEFINITION  RNAi cloning vector pRISE DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Kondo T, Inagaki S, Yasuda K, Kageyama Y.
  TITLE     Rapid construction of Drosophila RNAi transgenes using pRISE, a 
            P-element-mediated transformation vector exploiting an in vitro 
            recombination system
  JOURNAL   Genes Genet. Syst. 81 (2), 129-134 (2006)
  PUBMED    16755136
REFERENCE   2  (bases 1 to 12502)
  AUTHORS   Kondo T, Inagaki S, Kageyama Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2006) Takefumi Kondo, Nara Inst. Sci. Tech., Grad.
            Sch. Biol. Sci.; 8916-5 Takayama, Ikoma, Nara 630-0192, Japan 
            (E-mail:ta-kondo@bs.naist.jp, Tel:81-743-72-5554, 
            Fax:81-743-72-5559)
REFERENCE   3  (bases 1 to 12502)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12502)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Genet. Syst."; date: "2006"; volume: "81"; issue: "2"; pages: 
            "129-134"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUN-2006) Takefumi Kondo, Nara Inst. Sci. Tech., Grad. Sch. 
            Biol. Sci."; volume: " 8916-5 Takayama, Ikoma, Nara 630-0192, Japan 
            (E-mail:ta-kondo@bs.naist.jp, Tel:81-743-72-5554, Fax"; pages: 
            "81-743-72-5559"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..586
                     /label=P element 5' end
     misc_feature    complement(602..4728)
                     /label=mini-white
                     /note="mini-white"
     CDS             complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
                     3507..3780,4175..4246))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     polyA_signal    complement(4756..4890)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5161..5181)
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     intron          complement(5311..5376)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    5460..5584
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5609..5639
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             5693..6349
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             6694..6996
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(7040..7164)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    complement(7175..7321)
                     /label=fushi-tarazu intron
                     /note="fushi-tarazu intron"
     protein_bind    7344..7468
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(7512..7814)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(8159..8815)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(8869..8899)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(8924..9048)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(9098..9336)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    complement(9355..9449)
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_feature    9510..9742
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        10544..10648
                     /label=AmpR promoter
     CDS             10649..11506
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11680..12268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"