Basic Vector Information
- Vector Name:
- pRISE
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12502 bp
- Type:
- RNAi cloning vector
- Replication origin:
- ori
- Source/Author:
- Kondo T, Inagaki S, Yasuda K, Kageyama Y.
- Promoter:
- hsp70
pRISE vector Map
pRISE vector Sequence
LOCUS 40924_37088 12502 bp DNA circular SYN 18-DEC-2018
DEFINITION RNAi cloning vector pRISE DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12502)
AUTHORS Kondo T, Inagaki S, Yasuda K, Kageyama Y.
TITLE Rapid construction of Drosophila RNAi transgenes using pRISE, a
P-element-mediated transformation vector exploiting an in vitro
recombination system
JOURNAL Genes Genet. Syst. 81 (2), 129-134 (2006)
PUBMED 16755136
REFERENCE 2 (bases 1 to 12502)
AUTHORS Kondo T, Inagaki S, Kageyama Y.
TITLE Direct Submission
JOURNAL Submitted (08-JUN-2006) Takefumi Kondo, Nara Inst. Sci. Tech., Grad.
Sch. Biol. Sci.; 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
(E-mail:ta-kondo@bs.naist.jp, Tel:81-743-72-5554,
Fax:81-743-72-5559)
REFERENCE 3 (bases 1 to 12502)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 12502)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genes
Genet. Syst."; date: "2006"; volume: "81"; issue: "2"; pages:
"129-134"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(08-JUN-2006) Takefumi Kondo, Nara Inst. Sci. Tech., Grad. Sch.
Biol. Sci."; volume: " 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
(E-mail:ta-kondo@bs.naist.jp, Tel:81-743-72-5554, Fax"; pages:
"81-743-72-5559"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..12502
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..586
/label=P element 5' end
misc_feature complement(602..4728)
/label=mini-white
/note="mini-white"
CDS complement(join(1364..1978,2049..2180,2401..2716,2778..3432,
3507..3780,4175..4246))
/codon_start=1
/gene="white"
/product="Drosophila white gene eye color pigment"
/label=mini-white
/note="This is a modified version of the white gene lacking
part of the first intron."
/translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
polyA_signal complement(4756..4890)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(5161..5181)
/codon_start=1
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
intron complement(5311..5376)
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
protein_bind 5460..5584
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 5609..5639
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 5693..6349
/codon_start=1
/label=CmR
/note="chloramphenicol acetyltransferase"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 6694..6996
/codon_start=1
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(7040..7164)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
misc_feature complement(7175..7321)
/label=fushi-tarazu intron
/note="fushi-tarazu intron"
protein_bind 7344..7468
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(7512..7814)
/codon_start=1
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(8159..8815)
/codon_start=1
/label=CmR
/note="chloramphenicol acetyltransferase"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter complement(8869..8899)
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
protein_bind complement(8924..9048)
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(9098..9336)
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
protein_bind complement(9355..9449)
/label=5X UAS
/note="five tandem copies of the 'ScaI site' 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
misc_feature 9510..9742
/label=P element 3' end
/note="P element 3' end"
promoter 10544..10648
/label=AmpR promoter
CDS 10649..11506
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 11680..12268
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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