pRES19 vector (V003758)

LOCUS       V003758                 6140 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003758
VERSION     V003758
KEYWORDS    beta-galactosidase; beta-lactamase; rRNA methylase; rep protein
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Ishikawa J, Niino Y, Hotta K.
  TITLE     Construction of pRES18 and pRES19, Streptomyces-Escherichia coli
            shuttle vectors carrying multiple cloning sites
  JOURNAL   FEMS Microbiol. Lett. 145 (1), 113-116 (1996)
   PUBMED   8931335
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Ishikawa J, Sudoh M, Hotta K.
  TITLE     Nucleotide sequence of the pRES19
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6140)
  AUTHORS   Ishikawa J, Sudoh M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2000) Jun Ishikawa, National Institute of
            Infectious Diseases, Department of Bioactive Molecules; 1-23-1
            Toyama, Shinjuku, Tokyo 162-8640, Japan (E-mail:jun@nih.go.jp,
            URL:http://www.nih.go.jp/niid/index-e.html,
            Tel:81-3-5285-1111(ex.2354), Fax:81-3-5285-1272)
REFERENCE   4  (bases 1 to 6140)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6140)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett."; date: "1996"; volume: "145"; issue: "1"; pages:
            "113-116"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (22-MAR-2000) Jun Ishikawa, National Institute of Infectious
            Diseases, Department of Bioactive Molecules"; volume: " 1-23-1
            Toyama, Shinjuku, Tokyo 162-8640, Japan (E-mail:jun@nih.go.jp,
            URL:http://www.nih.go.jp/niid/index-e.html,
            Tel:81-3-5285-1111(ex.2354), Fax"; pages: "81-3-5285-1272"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      425..434
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1219..2025)
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     promoter        2703..2807
                     /label="AmpR promoter"
     CDS             2808..3665
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3839..4427
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4715..4736
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4751..4781
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4789..4805
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4813..4829
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(4842..4898)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4899..4915)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"