Basic Vector Information
- Vector Name:
- pRED56-MD8-16
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7889 bp
- Type:
- Gateway recycling vector
- Replication origin:
- ori
- Source/Author:
- Saisho T, Shibahara K, Nakao A, Tanaka Y, Nishimura K, Kimura T, Nakagawa T.
pRED56-MD8-16 vector Map
pRED56-MD8-16 vector Sequence
LOCUS 40924_36503 7889 bp DNA circular SYN 18-DEC-2018 DEFINITION Gateway recycling vector pRED56-MD8-16 DNA, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7889) AUTHORS Saisho T, Shibahara K, Nakao A, Tanaka Y, Nishimura K, Kimura T, Nakagawa T. TITLE Improved Gateway Recycling Cloning System for Multigene Construction JOURNAL Unpublished REFERENCE 2 (bases 1 to 7889) AUTHORS Saisho T, Shibahara K, Nakao A, Tanaka Y, Nishimura K, Kimura T, Nakagawa T. TITLE Direct Submission JOURNAL Submitted (02-NOV-2015) Contact:Tsuyoshi Nakagawa Shimane University, Interdisciplinary Center for Science Research; 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan REFERENCE 3 (bases 1 to 7889) TITLE Direct Submission REFERENCE 4 (bases 1 to 7889) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-NOV-2015) Contact:Tsuyoshi Nakagawa Shimane University, Interdisciplinary Center for Science Research; 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT constructed using pDONR201. FEATURES Location/Qualifiers source 1..7889 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 28..127 /label=attL1 /note="recombination site for the Gateway(R) LR reaction" misc_feature 674..1071 /label=MD8 /note="MD8" protein_bind 1094..1218 /label=attR1 /note="recombination site for the Gateway(R) LR reaction" promoter 1255..1285 /label=lac UV5 promoter /note="E. coli lac promoter with an 'up' mutation" CDS 1339..1995 /codon_start=1 /label=CmR /note="chloramphenicol acetyltransferase" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" CDS 2340..2642 /codon_start=1 /label=ccdB /note="CcdB, a bacterial toxin that poisons DNA gyrase" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" protein_bind complement(2686..2810) /label=attR2 /note="recombination site for the Gateway(R) LR reaction" CDS 2836..2865 /codon_start=1 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" /translation="EQKLISEEDL" CDS 2872..2901 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 2917..2946 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 2953..2982 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" terminator 3013..3265 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" protein_bind 3474..3598 /label=attR4 /note="recombination site for the Gateway(R) LR reaction" promoter 3623..3653 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" misc_feature 3925..3965 /label=rare cutter sites /note="rare cutter sites" CDS complement(4057..4914) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4915..5019) /label=AmpR promoter protein_bind complement(5316..5439) /label=attR3 /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(5765..5860) /label=attL6 /note="recombination site for the Gateway(R) LR reaction" CDS 5984..6790 /codon_start=1 /label=KanR /note="aminoglycoside phosphotransferase" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 6936..7524 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" terminator complement(7659..7686) /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator complement(7778..7864) /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene"
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