pRED-PT4exp vector (V003814)

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LOCUS       40924_36453       12675 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pRED-PT4exp, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12675)
  AUTHORS   Krajinski F.
  TITLE     A vector to express artificial microRNAs in Medicago truncatula
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12675)
  AUTHORS   Krajinski F.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2012) Max Planck Institute of Molecular Plant 
            Physiology, Am Muehlenberg 1, Potsdam (OT) Golm 14476, Germany
REFERENCE   3  (bases 1 to 12675)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JUL-2012) Max Planck Institute of Molecular Plant Physiology, Am
            Muehlenberg 1, Potsdam (OT) Golm 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        80..424
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             439..1239
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     regulatory      1255..1456
                     /regulatory_class="terminator"
     terminator      complement(1469..1721)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1737..2414)
                     /label=DsRed1
                     /note="wild-type DsRed"
     regulatory      complement(2428..3907)
                     /label=ubiquitin 10 promoter from Arabidopsis thaliana
                     /note="ubiquitin 10 promoter from Arabidopsis thaliana"
                     /regulatory_class="promoter"
     regulatory      3945..3954
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      complement(3956..4822)
                     /label=MtPt4 promoter from Medicago truncatula
                     /note="MtPt4 promoter from Medicago truncatula"
                     /regulatory_class="promoter"
     regulatory      complement(4870..5065)
                     /regulatory_class="terminator"
     misc_feature    5149..5173
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5818..6406
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6591..6731)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             8066..8692
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             9124..10194
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      10263..10457
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             11062..11850
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_feature    12373..12397
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"