pRD29A-GFP vector (V003817)

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LOCUS       40924_36433       14856 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRD29A-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Chen W-P.
  TITLE     Cre-lox recombination-based plant expression vector with 
            stress-inducible promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14856)
  AUTHORS   Chen W-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2005) Department of Horticultural Science, 
            Laboratory of Plant Hardiness, University of Minnesota, 1970 Folwell
            Avenue, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 14856)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAY-2005) Department of Horticultural Science, Laboratory of 
            Plant Hardiness, University of Minnesota, 1970 Folwell Avenue, St. 
            Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(826..850)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(1011..1211)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     primer_bind     complement(1244..1260)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3060..3648
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3936..3957
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3972..4002
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4010..4026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4034..4050
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4071..4089
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      4117..5065
                     /label=rd29A 1kb promoter
                     /note="rd29A 1kb promoter"
                     /regulatory_class="promoter"
     protein_bind    complement(5072..5105)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5108..5824
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      5898..6150
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6172..6188)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6221..6239)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6249..6265)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6407..6835
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        complement(6878..7258)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             7363..7911
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     primer_bind     complement(8178..8194)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8628..8758
                     /label=Agrobacterium tumefaciens Ti plasmid left border
                     /note="Agrobacterium tumefaciens Ti plasmid left border"
     misc_feature    complement(8812..8836)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10435..11580)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(11882..12673)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     mobile_element  12865..13632
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(14325..14856)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"