Basic Vector Information
- Vector Name:
- pRC-Niv-N
- Length:
- 4566 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Guo L-X., Chen J-M., Wang Z-L., Sun C-Y., Li L, Li J-M., Song C-P.
pRC-Niv-N vector Map
pRC-Niv-N vector Sequence
LOCUS V003824 4566 bp DNA circular SYN 18-DEC-2018 DEFINITION Exported. ACCESSION V003824 VERSION V003824 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 4566) AUTHORS Guo L-X., Chen J-M., Wang Z-L., Sun C-Y., Li L, Li J-M., Song C-P. TITLE Establishment of a Taqman real time RT-PCR assay for the detection of Nipah virus JOURNAL Unpublished REFERENCE 2 (bases 1 to 4566) AUTHORS Guo L-X., Chen J-M., Wang Z-L., Sun C-Y., Li L, Li J-M., Song C-P. TITLE Direct Submission JOURNAL Submitted (02-APR-2006) Chinese Center for Animal Health and Epidemiology, Nanjing Road 369, Qingdao, Shandong 266032, China REFERENCE 3 (bases 1 to 4566) TITLE Direct Submission REFERENCE 4 (bases 1 to 4566) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-APR-2006) Chinese Center for Animal Health and Epidemiology, Nanjing Road 369, Qingdao, Shandong 266032, China" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4566 /mol_type="other DNA" /organism="synthetic DNA construct" CDS 23..1618 /gene="N" /label="Nucleoprotein" /note="Nucleoprotein from Nipah virus. Accession#: Q9IK92" terminator 1728..1775 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 1872..2327 /label="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2408..2512 /label="AmpR promoter" misc_feature 2697..3075 /label="ISS" /note="immunostimulatory sequence from the AmpR gene; contains unmethylated CpG dinucleotides in the context of 5'-AACGTT-3' (Sato et al., 1996)" rep_origin 3544..4132 /direction=RIGHT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 4387..4405 /label="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" RBS 4436..4458 /label="RBS" /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 4478..4495 /label="6xHis" /note="6xHis affinity tag" CDS 4499..4531 /label="T7 tag (gene 10 leader)" /note="leader peptide from bacteriophage T7 gene 10" CDS 4535..4558 /label="Xpress(TM) tag" /note="Xpress(TM) epitope tag, including an enterokinase recognition and cleavage site"
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