pRC-Niv-N vector (V003824)

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LOCUS       V003824                 4566 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003824
VERSION     V003824
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Guo L-X., Chen J-M., Wang Z-L., Sun C-Y., Li L, Li J-M., Song C-P.
  TITLE     Establishment of a Taqman real time RT-PCR assay for the detection
            of Nipah virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Guo L-X., Chen J-M., Wang Z-L., Sun C-Y., Li L, Li J-M., Song C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2006) Chinese Center for Animal Health and
            Epidemiology, Nanjing Road 369, Qingdao, Shandong 266032, China
REFERENCE   3  (bases 1 to 4566)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-APR-2006) Chinese Center for Animal Health and Epidemiology,
            Nanjing Road 369, Qingdao, Shandong 266032, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             23..1618
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Nipah virus. Accession#: Q9IK92"
     terminator      1728..1775
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1872..2327
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2408..2512
                     /label="AmpR promoter"
     misc_feature    2697..3075
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      3544..4132
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4387..4405
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4436..4458
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4478..4495
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4499..4531
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             4535..4558
                     /label="Xpress(TM) tag"
                     /note="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"