pRAN334 vector (V003828)

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LOCUS       V003828                 7922 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003828
VERSION     V003828
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Ransom EM, Williams KB, Weiss DS, Ellermeier CD.
  TITLE     Identification and characterization of a gene cluster required for
            proper rod shape, cell division, and pathogenesis in Clostridium
            difficile
  JOURNAL   J. Bacteriol. 196 (12), 2290-2300 (2014)
   PUBMED   24727226
REFERENCE   2  (bases 1 to 7922)
  AUTHORS   Ransom EM, Weiss DS, Ellermeier CD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2015) Microbiology, The University of Iowa, 51
            Newton Rd, Iowa City, IA 52242, USA
REFERENCE   3  (bases 1 to 7922)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7922)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2014"; volume: "196"; issue: "12"; pages:
            "2290-2300"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-AUG-2015) Microbiology, The University of Iowa, 51 Newton Rd,
            Iowa City, IA 52242, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(58..681)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     protein_bind    788..806
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             873..1589
                     /label="ECFP"
                     /note="enhanced CFP"
     CDS             complement(1941..2309)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(2342..2451)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(2830..3450)
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      complement(3813..4401)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5194..6831)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label="repA"
                     /note="plasmid replication; derived from Clostridium
                     difficile"
                     /protein_id="AMA07794.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNSK
                     QLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINRYA
                     LGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVSEVT
                     NTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKKYCKD
                     VKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQVISVA
                     REWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIYSDEDTL
                     FNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITYKRKKKVK
                     NIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYTISYFVTMA
                     VIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQQRISNMIES
                     LLDTKILDVWETKINDRGFMYYTYRLNK"
     gene            complement(5194..6831)
                     /gene="repA"
                     /label="repA"
     CDS             complement(7106..7618)
                     /codon_start=1
                     /gene="orfB"
                     /product="OrfB"
                     /label="orfB"
                     /protein_id="AMA07792.1"
                     /translation="MRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNII
                     NDKLYYSNIAEFEEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNIT
                     KNGNTNYLKVKNKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSR
                     PSRVFLK"
     gene            complement(7106..7618)
                     /gene="orfB"
                     /label="orfB"