pRama-34 vector (V003830)

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LOCUS       V003830                 8361 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003830
VERSION     V003830
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Ganguly S, Liu J, Pillai VB, Mittler RS, Amara RR.
  TITLE     Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a
            HIV-1 Gag DNA vaccine: different effects on cellular and humoral
            immunity
  JOURNAL   Vaccine 28 (5), 1300-1309 (2010)
   PUBMED   19944789
REFERENCE   2  (bases 1 to 8361)
  AUTHORS   Ganguly S, Amara RR.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2009) Microbiology and Immunology, Emory
            University, 954 Gatewood Road NE, Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 8361)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8361)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine";
            date: "2010"; volume: "28"; issue: "5"; pages: "1300-1309"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUN-2009) Microbiology and Immunology, Emory University, 954
            Gatewood Road NE, Atlanta, GA 30329, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        93..472
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        476..675
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          814..1640
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             1650..3182
                     /gene="gag"
                     /label="Gag polyprotein"
                     /note="Gag polyprotein from Human immunodeficiency virus
                     type 1 group M subtype B (isolate BH10). Accession#:
                     P03347"
     polyA_signal    3225..3447
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      complement(3445..3479)
                     /label="Lambda To terminator"
                     /note="Lambda To terminator"
                     /regulatory_class="terminator"
     terminator      3448..3481
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     enhancer        3626..4007
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4008..4211
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          4224..5171
                     /number=A
                     /note="CMV intron A"
     intron          4347..5173
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             5292..6218
                     /gene="Tnfsf9"
                     /label="Tumor necrosis factor ligand superfamily member 9"
                     /note="Tumor necrosis factor ligand superfamily member 9
                     from Mus musculus. Accession#: P41274"
     regulatory      6225..6447
                     /label="bovine growth hormone polyadenylation signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    6228..6435
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     terminator      complement(6451..6485)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(6506..7297)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      7709..8297
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"