pRama-31 vector (V003831)

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LOCUS       V003831                 4828 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003831
VERSION     V003831
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Ganguly S, Liu J, Pillai VB, Mittler RS, Amara RR.
  TITLE     Adjuvantive effects of anti-4-1BB agonist Ab and 4-1BBL DNA for a
            HIV-1 Gag DNA vaccine: different effects on cellular and humoral
            immunity
  JOURNAL   Vaccine 28 (5), 1300-1309 (2010)
   PUBMED   19944789
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Ganguly S, Amara RR.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2009) Microbiology and Immunology, Emory
            University, 954 Gatewood Road NE, Atlanta, GA 30329, USA
REFERENCE   3  (bases 1 to 4828)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vaccine";
            date: "2010"; volume: "28"; issue: "5"; pages: "1300-1309"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUN-2009) Microbiology and Immunology, Emory University, 954
            Gatewood Road NE, Atlanta, GA 30329, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        93..474
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        475..678
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          814..1640
                     /label="CMV intron A"
                     /note="human cytomegalovirus intron A (Chapman et al.,
                     1991)"
     CDS             1759..2685
                     /gene="Tnfsf9"
                     /label="Tumor necrosis factor ligand superfamily member 9"
                     /note="Tumor necrosis factor ligand superfamily member 9
                     from Mus musculus. Accession#: P41274"
     polyA_signal    2695..2917
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     terminator      complement(2918..2952)
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2973..3764)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4176..4764
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"