praAqp4-Luc vector (V003836)

LOCUS       V003836                 6181 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003836
VERSION     V003836
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Holen T, Moe SE, Sorbo JG, Meza TJ, Ottersen OP, Klungland A.
  TITLE     Tolerated wobble mutations in siRNAs decrease specificity, but can
            enhance activity in vivo
  JOURNAL   Nucleic Acids Res. 33 (15), 4704-4710 (2005)
   PUBMED   16113241
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Holen T, Ottersen OP, Klungland A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2004) CMBN, University of Oslo, Sognsvannsveien 9,
            Oslo 0317, Norway
REFERENCE   3  (bases 1 to 6181)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6181)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res."; date: "2005"; volume: "33"; issue: "15"; pages:
            "4704-4710"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2004) CMBN, University of Oslo, Sognsvannsveien 9, Oslo
            0317, Norway"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..244
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     CDS             248..1207
                     /gene="Aqp4"
                     /label="Aquaporin-4"
                     /note="Aquaporin-4 from Rattus norvegicus. Accession#:
                     P47863"
     CDS             1205..2854
                     /label="luciferase"
                     /note="firefly luciferase"
     polyA_signal    complement(2898..3019)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3684..4272)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4446..5303)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5304..5408)
                     /label="AmpR promoter"
     rep_origin      5435..5890
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6021..6069
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    6083..6174
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"