pQTEV2 vector (V003844)

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LOCUS       40924_36263        4825 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQTEV2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ergin A, Thiel A, Duchmann R, Sieper J, Bussow K, Adam T.
  TITLE     Homologous High-Throughput Expression and Purification of Highly 
            Conserved E. coli Proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Ergin A, Adam T, Bussow K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2007) Gastroenterology, Charite Berlin (CBF), 
            Hindenburgdamm 30, Berlin 12200, Germany
REFERENCE   3  (bases 1 to 4825)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4825)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-FEB-2007) Gastroenterology, Charite Berlin (CBF), Hindenburgdamm
            30, Berlin 12200, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      101..107
                     /regulatory_class="ribosome_binding_site"
     misc_feature    115
                     /note="translation start site for inserts cloned into the 
                     multiple cloning site"
     CDS             121..141
                     /label=7xHis
                     /note="6xHis affinity tag"
     protein_bind    155..175
                     /label=attB1
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             178..198
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    195..230
                     /note="multiple cloning site; includes BamHI, SphI, NotI 
                     restriction sites"
     protein_bind    complement(236..260)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      282..376
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             420..1076
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1144..1230
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(1289..1310)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1326..2405)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(2406..2483)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    2675..2815
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3001..3589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3763..4620)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4621..4725)
                     /label=AmpR promoter