pQE30NST vector (V003857)

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LOCUS       40924_36178        3494 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQE30NST, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Bussow K, Cahill D, Nietfeld W, Bancroft D, Scherzinger E, Lehrach 
            H, Walter G.
  TITLE     A method for global protein expression and antibody screening on 
            high-density filters of an arrayed cDNA library
  JOURNAL   Nucleic Acids Res. 26 (21), 5007-5008 (1998)
  PUBMED    9776767
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Bussow K, Cahill D, Nietfeld W, Bancroft D, Scherzinger E, Lehrach 
            H, Walter G, Doebeli H, Eggimann B, Gentz R, Hochuli E, Stueber D.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-1998) Lehrach, Max-Planck-Institut fuer Molekulare
            Genetik, Ihnestrasse 73, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 3494)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3494)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998"; volume: "26"; issue: "21"; pages: 
            "5007-5008"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUN-1998) Lehrach, Max-Planck-Institut fuer Molekulare Genetik, 
            Ihnestrasse 73, Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      101..107
                     /regulatory_class="ribosome_binding_site"
     misc_feature    115
                     /note="translation start site for inserts cloned into the 
                     multiple cloning site"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     promoter        152..170
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(201..219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      240..334
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             378..1034
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1102..1188
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1344..1484
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1670..2258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2432..3289)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3290..3394)
                     /label=AmpR promoter