pP{white-STAR} vector (V003869)

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LOCUS       40924_35868        7237 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Targeting vector pP{white-STAR}, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Choi CM, Vilain S, Langen M, Van Kelst S, De Geest N, Yan J, 
            Verstreken P, Hassan BA.
  TITLE     Conditional mutagenesis in Drosophila
  JOURNAL   Science 324 (5923), 54 (2009)
  PUBMED    19342580
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Choi CM, Vilain S, Langen M, Van Kelst S, De Geest N, Yan J, 
            Verstreken P, Hassan BA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2009) Department of Molecular and Developmental 
            Genetics, VIB, Herestraat 49, Leuven 3000, Belgium
REFERENCE   3  (bases 1 to 7237)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7237)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science"; 
            date: "2009"; volume: "324"; issue: "5923"; pages: "54"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-FEB-2009) Department of Molecular and Developmental Genetics, 
            VIB, Herestraat 49, Leuven 3000, Belgium"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    25..42
                     /label=I-SceI site
                     /note="I-SceI site"
     protein_bind    43..76
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    complement(102..687)
                     /label=P element 5' end
     rep_origin      complement(922..1510)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1684..2541)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2542..2646)
                     /label=AmpR promoter
     misc_feature    complement(2731..2963)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3026..3059
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    3026..3038
                     /label=FRT
                     /note="FRT"
     misc_feature    3060..3077
                     /label=I-SceI site
                     /note="I-SceI site"
     misc_feature    3104..3153
                     /label=attP
                     /note="attP"
     CDS             join(3646..3717,4113..4385,4460..5110,5176..5490,5714..5843,
                     5914..6527)
                     /codon_start=1
                     /gene="white"
                     /product="white"
                     /label=white
                     /protein_id="ACN64944.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNVTADASQSCINQGFGQAKNYG
                     TLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERHI
                     PAPRKHLLKNGCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNGQ
                     PVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQELS
                     LSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQV
                     LKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSWVPSVLPT
                     TIRRTFTYRCWPLCPDGRSSPVIGSPRYGDNFAISKVARDMEQLLATKNLEKPLEQPEN
                     GYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTVAILIGLIFLGQQLTQVGVMN
                     INGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPLFLT
                     VPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSVGPP
                     VIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSSNTT
                     CPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     gene            3646..6527
                     /gene="white"
                     /label=white
     misc_feature    complement(7186..7235)
                     /label=attP
                     /note="attP"