pPZ3TA vector (V003879)

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LOCUS       V003879                14986 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003879
VERSION     V003879
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Goodwin TJ, Busby JN, Poulter RT.
  TITLE     A yeast model for target-primed (non-LTR) retrotransposition
  JOURNAL   BMC Genomics 8, 263 (2007)
   PUBMED   17683538
REFERENCE   2  (bases 1 to 14986)
  AUTHORS   Goodwin TJD., Busby JN, Poulter RTM.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUN-2007) Department of Biochemistry, University of
            Otago, Cumberland Street, Dunedin 9016, New Zealand
REFERENCE   3  (bases 1 to 14986)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14986)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC
            Genomics 8, 263 (2007)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-JUN-2007) Department of Biochemistry, University of Otago,
            Cumberland Street, Dunedin 9016, New Zealand"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      23..1043
                     /label="Candida albicans ACT1 promoter"
                     /note="Candida albicans ACT1 promoter"
                     /regulatory_class="promoter"
     repeat_region   1050..1069
                     /label="5' poly-A tract"
                     /note="5' poly-A tract"
     CDS             1075..2901
                     /codon_start=1
                     /product="Zorro3 ORF1 protein"
                     /label="Zorro3 ORF1 protein"
                     /protein_id="ABU62830.1"
                     /translation="MTTNGTNGFDFADKGDHPPRQSQENPPGNFQELPLDGGQIRKSSS
                     NDGVSNNVEFNTNTNAHESWADYPESDQMDTDSSEYYTEDENEEGKIPKGPYHQFQNAN
                     KFTYFTPETATTLVAPNNNTQNTTTEQDNMSKTYASVAATFKTNLAKSQDKQEELRMSN
                     FKVPQKAFDDLKRHNDAVFPEEVMTSDLSTIINKYGHEKVFDTIENTLITTAQNLEGYL
                     SEYPENVMRKAFKYFTLKLSIEDKQTRDVCEEILGIREELMYIQRILDFNIMDKVMCFM
                     PFTFEQMEGKLSSEKAKVRTIVENLELDFDGTVEHIKIDSAKRNRQLVAFKVPIHCRSL
                     AERYFRQIFLHKEDVIVQQYAPIIEKVQLGRKGKTEENNHQLLTEYVCYFIVDLFSGQV
                     PKRRIKIDNTKNKPSSYEQCTSRVLSNCKYCEYCRSMTHTKYHCPLIKPCTNCGVKGHK
                     TTSCKKALSTNTLVLKRPETKPNFPILPPKVVNKTTSDDSQRKGSIAKTTSHTKGDNTE
                     KMVTPSAQNENTTLQESIPVTITGHREAPTTPPVADTTAKKTMTTGVLEMDTSKHTISS
                     NDEASPRKKLNVKEKPSNPEKDPGLNLPPNLQ"
     CDS             3074..6523
                     /codon_start=1
                     /product="Zorro3 ORF2 protein"
                     /label="Zorro3 ORF2 protein"
                     /note="contains putative endonuclease and reverse
                     transcriptase domains"
                     /protein_id="ABU62831.1"
                     /translation="MKRNESYINSLTIGSKNIGSHQSTDFKKLLDIFLKLIGEHLMIDI
                     WFIQEIRFVSQEQFNYINKILKQHNAQLRMHHFEDLTGFLIHSPHAKLKFKIRDNDNHH
                     TTHFEGRISILDITLITNEDITLINNYLHSGNMDAQMTLLKAFIKYIANLKKHTNHNII
                     YGGDYNHIMLLDDVQLPLDQTRYIISKKELEIIQLMSNFYKKWKLQDAFQIRNNLQPTN
                     FHSNKSVKKRLDRIYIDSRIRRKLRNCRILEEFKQISTHKIIAMSFQIQKEPILKVGNP
                     RYLIPQWMSQDENIIKDLNNNEQSSLTPFSNWNGIINRIKEKVIFYEKYQRYIRAYIPH
                     AGNFPDEKMLKFRFRPSLNFSIITEMQTESGNTVQDTEIMINLATKFYQDLFLVEDRHL
                     ESFTFVEQFDKKIDDTDKVLLEKAFIEENVYDHLLMINKKTAVGTDGISYQNLIELWPS
                     LGESLIRAGNNILKYGTLPQQMSEVIITLIPKKLKTPIIENFRPISVISCAVRLLSSVI
                     EKQLNPVLAKVIEKTQTGFLKERSISNSIYLLDMVLTRYQTSKTADAESAGFINLDFRK
                     AFDTVHHDFILKVLQQVGFGPKATNFLMAITAKQKAKVSINNIEGPCFPLKRGVRQGNP
                     ISPLIFILILETFLARLSKEIEGIGVVNEVSLVAYTAYADDVIIFFKNKNDQERIQQLL
                     EDFGRESGLYLNNNKTEVCYFNDIPEISFLPYVSKKLQLEKLTYLGVPMKKADEEFDPW
                     TLFVLNLNAQIRMTPILDLPYQLIMKLMNIFIFSKLYYRDLHSPILTTAVSSIITTVQQ
                     RLPLYFKLQRLQTPNHLGGFGLMNPNHQVKGRRGKQIYLLYTQEDDLIIKFMRTKIQDI
                     LDNIAKDYYTMPTEPDKLIVYPWYLFGMGVSCHLSLQFRYLKERVYENLTKLEISWFEA
                     WFQLVHYTGPPVETPIIIMLIEDYANLIIQPQTESIKLMSPNFEEQSLSEQTFHHTSRK
                     LCPQAPIIPEGWGKHFDTIKSLTISDWTEFWKNMNKVQKQSVGSLQDYHLFILGYYSHY
                     PFYPNYKKLEIHFCQLCNTGTDSIVHHIFECGETMELWLKHFNSQRTPQFIIGNKHLHK
                     KDLYALNEYIKEVVQKVKRRRGSPILNQGERENVDAGTNVLV"
     CDS             complement(6649..7455)
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     intron          7450..7533
                     /note="intron from Candida albicans peptide transporter in
                     GenBank Accession Number U09781"
     regulatory      complement(7543..7677)
                     /label="Candida albicans URA3 promoter"
                     /note="Candida albicans URA3 promoter"
                     /regulatory_class="promoter"
     repeat_region   7828..7847
                     /label="3' poly-A tract"
                     /note="3' poly-A tract"
     misc_feature    7848..7998
                     /label="Zorro3 3' flanking sequence"
                     /note="Zorro3 3' flanking sequence"
     misc_feature    8004..8394
                     /note="ACT1 terminator; derived from Candida albicans"
     gene            complement(8401..11095)
                     /gene="IMH3"
                     /label="IMH3"
                     /note="derived from Candida albicans"
     CDS             complement(join(8685..9799,10047..10497))
                     /codon_start=1
                     /gene="IMH3"
                     /product="mycophenolic acid resistance protein"
                     /label="IMH3"
                     /protein_id="ABU62833.1"
                     /translation="MVFETSKATSYLKDYPKKDGLSVKELIDSTNFGGLTYNDFLILPG
                     LINFPSSAVSLETKLTKKITLKSPFVSSPMDTVTEENMAIHMALLGGIGIIHHNCTSEE
                     QAEMVRKVKKYENGFINDPVVISPEVTVGEVKKMGEVLGFTSFPVTENGKVGGKLVGII
                     TSRDIQFHEDNKSPVSEVMTKDLVVGKKGISLTDGNELLRSSKKGKLPIVDAEGNLVSL
                     ISRTDLQKNQDYPNASKSFHSKQLLCGATIGTIDADRERLDKLVEAGLDVVVLDSSNGS
                     SVFQLNMIKWIKEKYPELQVIAGNVVTREQAALLIEAGADALRIGMGSGSICITQEVMA
                     CGRPQGTAVYGVTEFANKFGVPCIADGGIGNIGHITKALALGASCVMMGGLLAGTAETP
                     GDYFYRDGKRLKTYRGMGSIDAMQQTNTNANASTSRYFSEADKVLVAQGVSGSVVDKGS
                     ITKFVPYLYNGLQHSLQDIGIKSIDELRENVDNGEIRFEFRTASAQFEGGVHGLHSYEK
                     RLHN"
     CDS             11385..12152
                     /gene="RPS1"
                     /label="Small ribosomal subunit protein eS1"
                     /note="Small ribosomal subunit protein eS1 from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P40910"
     primer_bind     complement(12370..12386)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    12394..12410
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12418..12448)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12463..12484)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12772..13360)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(13534..14391)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(14392..14496)
                     /label="AmpR promoter"
     primer_bind     14970..14986
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"