pPyr-Kan vector (V003880)

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LOCUS       40924_35753        5444 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pPyr-Kan, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Middleton R, Hofmeister A.
  TITLE     New shuttle vectors for ectopic insertion of genes into Bacillus 
            subtilis
  JOURNAL   Plasmid 51 (3), 238-245 (2004)
  PUBMED    15109830
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Middleton RH, Hofmeister AEM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2003) Plant and Microbial Biology, University of 
            California, Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 5444)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5444)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2004"; volume: "51"; issue: "3"; pages: "238-245"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2003) Plant and Microbial Biology, University of California,
            Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..495)
                     /note="3' end of dihydroorotate dehydrogenase (catalytic 
                     subunit) (pyrD) of Bacillus subtilis"
     CDS             1467..2258
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    complement(2714..3133)
                     /note="5' end of dihydroorotate dehydrogenase (pyrD) of
                     Bacillus subtilis"
     promoter        3538..3642
                     /label=AmpR promoter
     CDS             3643..4500
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4674..5262
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"