pPYPX99 vector (V003882)

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LOCUS       40924_35743        9139 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPYPX99, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Yao Q, Peng R, Xiong A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2002) Shanghai Academy of Agricultural Sciences, 
            Agro-Biotechnology Research Center, 2901 Beidi Rd, Shanghai 201106, 
            China
REFERENCE   2  (bases 1 to 9139)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9139)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (12-NOV-2002) Shanghai Academy of Agricultural Sciences, 
            Agro-Biotechnology Research Center, 2901 Beidi Rd, Shanghai 201106, 
            China"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     NCBI staff are still waiting for submitters to provide appropriate 
            coding region information.
FEATURES             Location/Qualifiers
     source          1..9139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..936
                     /label=modified AOX1 promoter
                     /note="modified AOX1 promoter"
     misc_feature    937..1212
                     /label=similar to modified alpha factor secretion signal
                     /note="similar to modified alpha factor secretion signal"
     terminator      1343..1589
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     misc_feature    complement(1994..4527)
                     /label=similar to phosphoribosyl-ATP pyrophosphohydrolase
                     /note="similar to phosphoribosyl-ATP pyrophosphohydrolase"
     CDS             4798..5610
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     misc_feature    5985..6741
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    6884..7024
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7210..7798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7972..8829)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8830..8934)
                     /label=AmpR promoter
     promoter        9139
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"