Basic Vector Information
- Vector Name:
- pPV570
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4596 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Lok JB, Shao H, Massey HC, Li X.
pPV570 vector Map
pPV570 vector Sequence
LOCUS 40924_35693 4596 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pPV570, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4596) AUTHORS Lok JB, Shao H, Massey HC, Li X. TITLE Transgenesis in Strongyloides and related parasitic nematodes: historical perspectives, current functional genomic applications and progress towards gene disruption and editing JOURNAL Parasitology (2016) In press PUBMED 27000743 REFERENCE 2 (bases 1 to 4596) AUTHORS Lok JB, Shao H, Massey HC Jr., Li X. TITLE Direct Submission JOURNAL Submitted (02-FEB-2016) Pathobiology, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, USA REFERENCE 3 (bases 1 to 4596) TITLE Direct Submission REFERENCE 4 (bases 1 to 4596) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Parasitology (2016) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2016) Pathobiology, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4596 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 24..128 /label=AmpR promoter CDS 129..986 /label=AmpR /note="beta-lactamase" rep_origin 1160..1748 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2036..2057 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2072..2102 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2110..2126 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2134..2150 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" regulatory 2956..3467 /note="U6; Strogyloides ratti promoter" /regulatory_class="promoter" misc_feature 3467..3489 /label=gRNA for Cherry target /note="gRNA for Cherry target" misc_RNA 3492..3567 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" primer_bind 3576..3592 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3981..3997) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 4139..4594 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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