Basic Vector Information
- Vector Name:
- pPV254
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6758 bp
- Type:
- PiggyBac_based_Ss_Act2_gfp_era1 vector
- Replication origin:
- ori
- Source/Author:
- Shao H, Li X, Nolan TJ, Massey HC Jr., Pearce EJ, Lok JB.
pPV254 vector Vector Map
pPV254 vector Sequence
LOCUS 40924_35658 6758 bp DNA circular SYN 18-DEC-2018 DEFINITION PiggyBac_based_Ss_Act2_gfp_era1 vector pPV254, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6758) AUTHORS Shao H, Li X, Nolan TJ, Massey HC Jr., Pearce EJ, Lok JB. TITLE Transposon-mediated Chromosomal Integration of Transgenes in the Parasitic Nematode Strongyloides ratti and Establishment of Stable Transgenic Lines JOURNAL PLoS Pathog. 8 (8), E1002871 (2012) PUBMED 22912584 REFERENCE 2 (bases 1 to 6758) AUTHORS Shao H, Li X, Nolan TJ, Massey HC Jr., Pearce EJ, Lok JB. TITLE Direct Submission JOURNAL Submitted (03-MAY-2012) Pathobiology, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, USA REFERENCE 3 (bases 1 to 6758) TITLE Direct Submission REFERENCE 4 (bases 1 to 6758) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Pathog."; date: "2012"; volume: "8"; issue: "8"; pages: "E1002871" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (03-MAY-2012) Pathobiology, University of Pennsylvania, 3800 Spruce Street, Philadelphia, PA 19104, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6758 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 24..128 /label=AmpR promoter CDS 129..986 /label=AmpR /note="beta-lactamase" rep_origin 1160..1748 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2036..2057 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2072..2102 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2110..2126 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2134..2150 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" mobile_element 2641..2675 /mobile_element_type="transposon:piggyBac ITR" /label=ITR CDS complement(3315..3326) /label=Factor Xa site /note="Factor Xa recognition and cleavage site" misc_recomb 3826..3830 /gene="act2" /label=target sequence /note="target sequence" CDS join(4249..4419,4471..4624,4676..4839,4891..5118) /codon_start=1 /product="GFP" /label=GFP /protein_id="AFN89784.1" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" 3'UTR 5138..5724 /gene="era1" gene 5138..5724 /gene="era1" /label=era1 /note="Ss-era1" primer_bind 5738..5754 /label=SK primer /note="common sequencing primer, one of multiple similar variants" mobile_element 5951..6013 /mobile_element_type="transposon:piggyBac ITR" /label=ITR misc_recomb 6014..6017 /label=target sequence /note="target sequence" primer_bind complement(6143..6159) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 6301..6756 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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