pPtPBR1 vector (V003907)

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LOCUS       40924_35608        7979 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPtPBR1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Diner RE, Bielinski VA, Dupont CL, Allen AE, Weyman PD.
  TITLE     Refinement of the Diatom Episome Maintenance Sequence and 
            Improvement of Conjugation-Based DNA Delivery Methods
  JOURNAL   Front Bioeng Biotechnol 4, 65 (2016)
  PUBMED    27551676
REFERENCE   2  (bases 1 to 7979)
  AUTHORS   Diner RE, Weyman PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2016) Scripps Institution of Oceanography, 
            University of California San Diego, 9500 Gilman Drive, La Jolla, CA 
            92093, USA
REFERENCE   3  (bases 1 to 7979)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7979)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol 4, 65 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUL-2016) Scripps Institution of Oceanography, University of 
            California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     regulatory      complement(44..49)
                     /label=P1 promoter
                     /note="P1 promoter"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(64..75)
                     /label=P1 promoter -35 sequence region
                     /note="P1 promoter -35 sequence region"
                     /regulatory_class="minus_35_signal"
     CDS             86..1273
                     /label=TcR
                     /note="tetracycline efflux protein"
     regulatory      1905..1909
                     /label=rop RBS
                     /note="rop RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             1915..2103
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(2340..2708)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(2741..2850)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     3127..3143
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3167..3185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3889..4260
                     /label=BleoR
                     /note="antibiotic-binding protein"
     misc_feature    4569..5072
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        5073..5259
                     /label=HIS3 promoter
     CDS             5260..5919
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     rep_origin      complement(6152..6740)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6914..7771)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7772..7876)
                     /label=AmpR promoter