pPRV312L vector (V003915)

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LOCUS       40924_35544        6348 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plastid transformation vector pPRV312L, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Chakrabarti SK, Lutz KA, Lertwiriyawong B, Svab Z, Maliga P.
  TITLE     Expression of the cry9Aa2 B.t. gene in tobacco chloroplasts confers 
            resistance to potato tuber moth
  JOURNAL   Transgenic Res. 15 (4), 481-488 (2006)
  PUBMED    16906448
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Maliga P, Lutz KA.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2006) Waksman Institute, Rutgers University, 190 
            Frelinghuysen Road, Piscataway, NJ 08854-8020, USA
REFERENCE   3  (bases 1 to 6348)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6348)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res."; date: "2006"; volume: "15"; issue: "4"; pages: "481-488"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2006) Waksman Institute, Rutgers University, 190 
            Frelinghuysen Road, Piscataway, NJ 08854-8020, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1242
                     /note="similar to Nicotiana tabacum ptDNA in GenBank
                     Accession Number Z00044; plastid sequence targeting 
                     insertions in the trnI-trnA intergenic region"
     misc_feature    1259..1315
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    1332..1365
                     /label=P1 phage loxP
                     /note="P1 phage loxP"
     protein_bind    1332..1365
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    1372..1544
                     /note="tobacco plastid rRNA operon promoter (Prrn) fused
                     with tobacco plastid atpB gene leader (described in Kuroda 
                     and Maliga, Plant Phys 125:430-436, 2001)"
     regulatory      1372..1455
                     /label=tobacco plastid rRNA operon promoter (Prrn)
                     /note="tobacco plastid rRNA operon promoter (Prrn)"
                     /regulatory_class="promoter"
     misc_feature    1455..1544
                     /label=tobacco plastid atpB gene leader
                     /note="tobacco plastid atpB gene leader"
     misc_feature    1545..1586
                     /gene="atpB-aad-c-myc"
                     /label=N-terminus of tobacco plastid AtpB
                     /note="N-terminus of tobacco plastid AtpB"
     CDS             1596..2378
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             2379..2408
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     misc_feature    2425..2613
                     /note="similar to tobacco plastid psbA 3' UTR in GenBank 
                     Accession Number Z00044"
     protein_bind    complement(2632..2665)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2674..2681
                     /label=contains SwaI restriction site
                     /note="contains SwaI restriction site"
     misc_feature    2685..3508
                     /note="similar to Nicotiana tabacum ptDNA in GenBank
                     Accession Number Z00044; plastid sequence targeting 
                     insertions in the trnI-trnA intergenic region"
     rep_origin      3651..4106
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4388..4492
                     /label=AmpR promoter
     CDS             4493..5350
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5524..6112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"