pPROEX-1 vector (V003918)

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LOCUS       40924_35539        4733 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPROEX-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Polayes D.
  TITLE     Plasmid pPROEX - cloning vector for protein expression and 
            purification
  JOURNAL   Unpublished
REFERENCE   2  (bases 2564 to 2571)
  AUTHORS   Polayes D, Hughes AJohn.
  TITLE     Efficient protein expression and simple purification using the 
            pPROEX-1 system
  JOURNAL   Focus 16, 81-84 (1994)
REFERENCE   3  (bases 1 to 4733)
  AUTHORS   Horton ME.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-1995) Mary E. Horton, Technical Services, Life 
            Technologies, Inc., 8400 Helgerman Court, Gaithersburg, MD 
            20884-9980, USA
REFERENCE   4  (bases 1 to 4733)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4733)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Focus 16, 
            81-84 (1994)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-1995) Mary E. Horton, Technical Services, Life Technologies,
            Inc., 8400 Helgerman Court, Gaithersburg, MD 20884-9980, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    230..246
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             273..290
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             312..332
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     terminator      625..711
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      803..830
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        850..941
                     /label=AmpR promoter
     CDS             942..1799
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1973..2561
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2564..2571
                     /standard_name="ORI Escherichia coli pMB1"
                     /label=ORI Escherichia coli pMB1
                     /citation=[2]
                     /experiment="experimental evidence, no additional details
                     recorded"
     rep_origin      complement(2809..3237)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3534..3611
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3612..4691
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"