pPROBE-NI vector (V003921)

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LOCUS       V003921                 9686 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003921
VERSION     V003921
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9686)
  AUTHORS   Miller WG, Leveau JH, Lindow SE.
  TITLE     Improved gfp and inaZ broad-host-range promoter-probe vectors
  JOURNAL   Mol. Plant Microbe Interact. 13 (11), 1243-1250 (2000)
   PUBMED   11059491
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   Leveau JHJ., Miller WG, Lindow SE.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9686)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant
            Microbe Interact."; date: "2000"; volume: "13"; issue: "11"; pages:
            "1243-1250"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-JUL-2000) Plant "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             1793..2452
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     primer_bind     3162..3178
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3188..3206
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3247..3333
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      3420..3592
                     /label="T1 rrnB terminator region"
                     /note="T1 rrnB terminator region"
                     /regulatory_class="terminator"
     terminator      3428..3514
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      3601..3773
                     /label="T1 rrnB terminator region"
                     /note="T1 rrnB terminator region"
                     /regulatory_class="terminator"
     terminator      3609..3695
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      3782..3954
                     /label="T1 rrnB terminator region"
                     /note="T1 rrnB terminator region"
                     /regulatory_class="terminator"
     terminator      3790..3876
                     /gene="Escherichia coli rrnB"
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    complement(3968..4024)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     regulatory      4047..4051
                     /label="putative"
                     /note="putative"
                     /regulatory_class="ribosome_binding_site"
     CDS             4059..7658
                     /gene="inaZ"
                     /label="Ice nucleation protein"
                     /note="Ice nucleation protein from Pseudomonas syringae pv.
                     syringae. Accession#: P06620"
     terminator      complement(7682..7725)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        complement(7877..7895)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7916..7932)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7940..7956)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7964..7994)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8009..8030)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(8438..9229)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"