pPRCG vector (V003931)

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LOCUS       V003931                 9479 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003931
VERSION     V003931
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Wu G, Wang Y, Zhu Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2004) Fish Genetics, Institute of Hydrobiology,
            Chinese Academy of Science, 7# South Road of East Lake, Wuhan, Hubei
            430072, People's Republic of China
REFERENCE   2  (bases 1 to 9479)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9479)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (09-MAR-2004) Fish Genetics, Institute of Hydrobiology, Chinese
            Academy of Science, 7# South Road of East Lake, Wuhan, Hubei 430072,
            People's Republic of China"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(419..452)
                     /label="lox71"
                     /note="Left element (LE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      459..2499
                     /label="salmon protamine promoter"
                     /note="salmon protamine promoter"
                     /regulatory_class="promoter"
     regulatory      823..832
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2506..2526
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2536..5184
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     polyA_signal    5366..5487
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     enhancer        5597..5900
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5901..6104
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             6212..6928
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    7054..7175
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      7085..7135
                     /label="SV40 polyA"
                     /note="SV40 polyA"
                     /regulatory_class="terminator"
     protein_bind    complement(7188..7221)
                     /label="lox66"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7258..7274)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7282..7298)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7306..7336)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7351..7372)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7660..8248)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8422..9279)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9280..9384)
                     /label="AmpR promoter"