pPR9TT vector (V003932)

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LOCUS       40924_35454        9388 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter probe vector pPR9TT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Santos PM, Blatny JM, Di Bartolo I, Valla S, Zennaro E.
  TITLE     Physiological analysis of the expression of the styrene degradation 
            gene cluster in Pseudomonas fluorescens ST
  JOURNAL   Appl. Environ. Microbiol. 66 (4), 1305-1310 (2000)
  PUBMED    10742204
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Santos PM, Di Bartolo I, Blatny JM, Zennaro E, Valla S.
  TITLE     New broad-host-range promoter probe vectors based on the plasmid RK2
            replicon
  JOURNAL   FEMS Microbiol. Lett. 195 (1), 91-96 (2001)
  PUBMED    11167001
REFERENCE   3  (bases 1 to 9388)
  AUTHORS   Santos PM, Blatny JM, Di Bartolo I, Valla S, Zennaro E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1999) Department of Biology, Third University of 
            Rome, Viale Marconi 446, Rome 00146, Italy
REFERENCE   4  (bases 1 to 9388)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9388)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2000"; volume: "66"; issue: "4"; pages:
            "1305-1310"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2001"; volume: "195"; issue: "1"; pages: 
            "91-96"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-1999) Department of Biology, Third University of Rome, Viale
            Marconi 446, Rome 00146, Italy"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(67..175)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        352..456
                     /label=AmpR promoter
     CDS             457..1314
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1564..2094
                     /label=oriV
                     /note="incP origin of replication"
     regulatory      2316..2496
                     /label=Pneo
                     /note="Pneo"
                     /regulatory_class="promoter"
     CDS             2486..3631
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     primer_bind     complement(3815..3831)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4327..4983
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      5150..5724
                     /label=bidirectional
                     /note="bidirectional"
                     /regulatory_class="terminator"
     primer_bind     5775..5791
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5813..8857)
                     /label=lacZ
                     /note="beta-galactosidase"
     primer_bind     complement(8897..8913)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(8964..8991)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(9083..9169)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"