pPOP vector (V003954)

Download GeneBank File(.gb)

LOCUS       40924_35339        5544 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPOP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Soderholm JF, Bevis BJ, Glick BS.
  TITLE     A vector for Pop-in/Pop-out gene replacement in Pichia pastoris
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Soderholm JF, Bevis BJ, Glick BS.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2000) Molecular Genetics and Cell Biology, 
            University of Chicago, 920 E. 58th St., Chicago, IL 60637, USA
REFERENCE   3  (bases 1 to 5544)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5544)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-NOV-2000) Molecular Genetics and Cell Biology, University of 
            Chicago, 920 E. 58th St., Chicago, IL 60637, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(302..886)
                     /codon_start=1
                     /product="T-urf13"
                     /label=T-urf13
                     /protein_id="AAK19282.1"
                     /translation="MASTRVLASRLASQMAASAKVARPAVRVAQVSKRTIQTGSPLQTL
                     KRTQMTSIVNATTRQAFQKRAYSSEIAQAMARSGMITTFLNLPPFDQGLVFFGSIFIFF
                     LCILLIKGYLRKMDDSYLAQLSELANHNRVEAAKAGHVALHELSFSWLRGVQIRVRTLP
                     IQWNEGGGRSNDQSTLSKPKYSSMTDSVQVP"
     promoter        complement(900..1376)
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     primer_bind     1585..1601
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1602..1658
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1671..1687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1695..1711)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1719..1749)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1764..1785)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2073..2661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2835..3692)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        3710..4025
                     /label=ARG4 promoter
     CDS             4026..5414
                     /codon_start=1
                     /label=ARG4
                     /note="argininosuccinate lyase, required for arginine 
                     biosynthesis"
                     /translation="MSDGTQKLWGGRFTGETDPLMHLYNASLPYDYKMYKADLEGTKVY
                     TAGLQKLGLLTETELAKIHEGLAEIKKEWDADKFVRHPNDEDIHTANERRLGELIGREI
                     AGKVHTGRSRNDQVVTDLRIYCRDIVNDTLFPALKGLVEVLIKRAEGEIDVLMPGYTHL
                     QRAQPIRWSHWLSSYATYFTEDYKRLGQILHRLNQSPLGAGALAGHPYGIDREFLAEGL
                     GFNSVIGNSLVAVSDRDFIVELMFWGTLFMNHISRFAEDLIIYCTAEFGFIQLSDAYST
                     GSSLMPQKKNADSLELLRGKSGRVFGDLTGFLMSLKGIPSTYDKDMQEDKEPLFDCLTT
                     VEHSMLIATGVISTLTVNKEKMEAALTMDMLATDLADYLVRKGVPFRETHHISGECVAT
                     AERLGLSGIDKLTLEQYQKIDSRFGQDLFETFNFEQSVERRDATGGTAKSAVLKQLDNL
                     KSQLN"