pPolyREP vector (V003956)

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LOCUS       V003956                 7403 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003956
VERSION     V003956
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Kolkenbrock S, Moerschbacher BM.
  TITLE     pPolyREPII, a Synthetic, Modular, Broad-Host-Range Expression Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Kolkenbrock S, Moerschbacher BM.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2013) Institute of Plant Biology and
            Biotechnology, WWU Muenster, Schlossplatz 8, Muenster, NRW 48143,
            Germany
REFERENCE   3  (bases 1 to 7403)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7403)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-SEP-2013) Institute of Plant Biology and Biotechnology, WWU
            Muenster, Schlossplatz 8, Muenster, NRW 48143, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        125..153
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    161..177
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      269..296
                     /label="trpA terminator"
                     /note="trpA terminator"
                     /regulatory_class="terminator"
     regulatory      305..332
                     /label="trpA terminator"
                     /note="trpA terminator"
                     /regulatory_class="terminator"
     regulatory      333..362
                     /label="t500 terminator"
                     /note="t500 terminator"
                     /regulatory_class="terminator"
     CDS             complement(1083..1094)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     regulatory      2559..2593
                     /label="tR2 terminator"
                     /note="tR2 terminator"
                     /regulatory_class="terminator"
     regulatory      2603..2636
                     /label="tR2 terminator"
                     /note="tR2 terminator"
                     /regulatory_class="terminator"
     CDS             complement(2657..3304)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     rep_origin      3790..4559
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
     CDS             4560..5219
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     terminator      5838..5919
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      6011..6038
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(6047..7126)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(7127..7204)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     regulatory      7292..7322
                     /label="hisT terminator"
                     /note="hisT terminator"
                     /regulatory_class="terminator"
     regulatory      7349..7389
                     /label="hisT terminator"
                     /note="hisT terminator"
                     /regulatory_class="terminator"