pPLc28LIG8 vector (V004034)

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LOCUS       V004034                 4708 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004034
VERSION     V004034
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4708)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4708)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..537
                     /label="5' UTR of phage T4 ligase, incl. RBS"
                     /note="5' UTR of phage T4 ligase, incl. RBS"
     CDS             538..1998
                     /gene="30"
                     /label="DNA ligase"
                     /note="DNA ligase from Enterobacteria phage T4. Accession#:
                     P00970"
     misc_feature    2151..2291
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2477..3065)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3239..4096)
                     /label="AmpR"
                     /note="beta-lactamase"
     regulatory      4462..4592
                     /label="PL promoter"
                     /note="PL promoter"
                     /regulatory_class="promoter"