Basic Vector Information
- Vector Name:
- pPlat-Pds-Mod4.1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8742 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Steinbrenner J, Sandmann G.
pPlat-Pds-Mod4.1 vector Map
pPlat-Pds-Mod4.1 vector Sequence
LOCUS 40924_34924 8742 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pPlat-Pds-Mod4.1, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8742) AUTHORS Steinbrenner J, Sandmann G. TITLE Transformation of the green alga Haematococcus pluvialis with a phytoene desaturase for accelerated astaxanthin biosynthesis JOURNAL Appl. Environ. Microbiol. 72 (12), 7477-7484 (2006) PUBMED 17012596 REFERENCE 2 (bases 1 to 8742) AUTHORS Steinbrenner J, Sandmann G. TITLE Direct Submission JOURNAL Submitted (16-FEB-2006) Plant Physiology and Biochemistry, University of Konstanz, Universitaetsstr. 10, Konstanz 78457, Germany REFERENCE 3 (bases 1 to 8742) TITLE Direct Submission REFERENCE 4 (bases 1 to 8742) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl. Environ. Microbiol."; date: "2006"; volume: "72"; issue: "12"; pages: "7477-7484" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (16-FEB-2006) Plant Physiology and Biochemistry, University of Konstanz, Universitaetsstr. 10, Konstanz 78457, Germany" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT Name: pPlat-Pds-Mod4.1. FEATURES Location/Qualifiers source 1..8742 /mol_type="other DNA" /organism="synthetic DNA construct" gene 134..5825 /gene="pdsMod4.1" /label=pdsMod4.1 regulatory 134..2140 /gene="pdsMod4.1" /label=derived from the phytoene desaturase gene /note="derived from the phytoene desaturase gene" /regulatory_class="promoter" mRNA join(2141..2510,2600..2712,2818..3184,3537..3671,3965..4043, 4394..4743,4974..5044,5222..5825) /gene="pdsMod4.1" /product="phytoene desaturase" /label=pdsMod4.1 mRNA CDS join(2181..2510,2600..2712,2818..3184,3537..3671,3965..4043, 4394..4743,4974..5044,5222..5489) /codon_start=1 /gene="pdsMod4.1" /product="phytoene desaturase" /label=pdsMod4.1 /protein_id="ABG29431.1" /translation="MQTTMRGQASGSGCTSGRQARGHWSRRSVRERGALRVVAKDYPTP DFQSSDTYQEALSLSTKLRNAPRPAKPLRVVIAGAGLAGLSAAKYLADAGHHPVVLEGR DVLGGKVAAWKDEDGDWYETGLHIFFGAYPNIQNLFKELGIQDRLQWKEHSMIFAMPDA PGEFSRFDFPELPAPWNGIIAILRNNQMLSWPEKIRFRIGLLPAIIFGQRYCEEQDELT VTEWMRKQGVPDRVNEEVFIAMAKALNFINPDDLSMTVVLTALNRFLQEQHGSKMAFLD GAPPERLCQPMVDYFKARGGDLMFNSRVKQIVLNDDKSVKHLALTNGQTVEGDLYISAM PVDIMKILMPDPWASMPYFKQLNGLEGVPVINIHIWFDRKLTTVDHLLFSRSPLLSVYA DMSTTSKEYRDDKKSMLELVFAPAKEWIGRPDEEIIAATMTELERLFPTEVRADQSMAK ILKYKVVKTPLSVYKSTAGREKFRPTQRSPISNFYLAGDYTKQKYRASMEGAVFSGKLV TEAIVEDWSARGVTSSAASRQPALAAAGVVGRVGSSDWARWLQPVGAIAGGCK" primer_bind 6387..6403 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 6410..6428 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature complement(6437..6544) /label=MCS /note="pBluescript multiple cloning site" promoter complement(6557..6575) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(6596..6612) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(6620..6636) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6644..6674) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(6689..6710) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(6998..7586) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(7760..8617) /label=AmpR /note="beta-lactamase" promoter complement(8618..8722) /label=AmpR promoter
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