pPLaR1 vector (V004038)

Basic Vector Information

Vector Name:
pPLaR1
Antibiotic Resistance:
Kanamycin
Length:
5258 bp
Type:
Bacterial expression vector
Replication origin:
ori
Source/Author:
De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.

pPLaR1 vector Map

pPLaR15258 bp6001200180024003000360042004800PLKanRoriIS1Lysis protein

pPLaR1 vector Sequence

LOCUS       V004038                 5258 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004038
VERSION     V004038
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5258)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5258)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(117..247)
                     /label="PL"
                     /note="PL"
                     /regulatory_class="promoter"
     CDS             615..1427
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1889..2477)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2965..3154)
                     /label="IS1"
                     /note="IS1"
     CDS             complement(4987..5211)
                     /note="Lysis protein from Escherichia phage MS2.
                     Accession#: P03609"
                     /label="Lysis protein"

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