pPL450 vector (V004051)

LOCUS       40924_34874        4237 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPL450 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Love CA, Lilley PE, Dixon NE.
  TITLE     Stable high-copy-number bacteriophage lambda promoter vectors for 
            overproduction of proteins in Escherichia coli
  JOURNAL   Gene 176 (1-2), 49-53 (1996)
  PUBMED    8918231
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Yasuda S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2006) Contact:Seiichi Yasuda National Institute of
            Genetics, Microbial Genetics; Yata 1111, Mishima, Shizuoka 411-8540,
            Japan
REFERENCE   3  (bases 1 to 4237)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4237)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 176 
            (1-2), 49-53 (1996)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-FEB-2006) Contact:Seiichi Yasuda National Institute of Genetics,
            Microbial Genetics; Yata 1111, Mishima, Shizuoka 411-8540, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    53..419
                     /label=partition(pSC101)
                     /note="partition(pSC101)"
     promoter        474..578
                     /label=AmpR promoter
     CDS             579..1436
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1610..2198
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2486..2507
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2522..2552
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2560..2576
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2584..2600
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2859..3569)
                     /label=lambda repressor
                     /note="phage lambda repressor"
     regulatory      3617..3644
                     /label=lambda PR
                     /note="lambda PR"
                     /regulatory_class="promoter"
     RBS             3658..3666
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      3831..3859
                     /label=lambda PL
                     /note="lambda PL"
                     /regulatory_class="promoter"
     misc_feature    3980..4072
                     /label=polylinker
                     /note="polylinker"
     primer_bind     complement(4080..4096)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"