Basic Vector Information
- Vector Name:
- pPKm-248
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11558 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Jian-Yu V, Chao SS, Hsu A, Pham V, Naghavian L, Dozier LE, Patrick G, Coleman T.
- Promoter:
- EF-1α
pPKm-248 vector Map
pPKm-248 vector Sequence
LOCUS V004054 11558 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V004054
VERSION V004054
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 11558)
AUTHORS Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Jian-Yu V, Chao
SS, Hsu A, Pham V, Naghavian L, Dozier LE, Patrick G, Coleman T.
TITLE Biosynthesis of Orthogonal Molecules Using Ferredoxin and
Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB
Optogenetics
JOURNAL ACS Synth Biol (2018) In press
PUBMED 29301067
REFERENCE 2 (bases 1 to 11558)
AUTHORS Kyriakakis P.
TITLE Direct Submission
JOURNAL Submitted (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive,
PFBH room 251 MC0412, La Jolla, CA 92093, United States of America
REFERENCE 3 (bases 1 to 11558)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11558)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
Biol (2018) In press"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive, PFBH room 251
MC0412, La Jolla, CA 92093, United States of America"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..11558
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..1179
/label="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
CDS 1264..1338
/label="COX8 presequence"
/note="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
misc_feature 1351..2061
/label="tPCYA"
/note="tPCYA"
misc_feature 2092..2718
/label="IRES"
/note="IRES"
CDS 2731..2805
/label="COX8 presequence"
/note="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
CDS 3553..3609
/label="P2A"
/note="2A peptide from porcine teschovirus-1 polyprotein"
misc_feature 3610..3696
/label="transit peptide mito"
/note="transit peptide mito"
CDS 3610..3684
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label="COX8 presequence"
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
regulatory 3706..3715
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3715..3756
/label="V5 tag"
/note="epitope tag from simian virus 5"
CDS 3763..4056
/gene="petF1"
/label="Ferredoxin-1"
/note="Ferredoxin-1 from Synechococcus elongatus.
Accession#: P0A3D0"
misc_feature 4057..4122
/label="P2A"
/note="P2A"
CDS 4066..4122
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label="P2A"
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
misc_feature 4123..4209
/label="transit peptide mito(2)"
/note="transit peptide mito(2)"
CDS 4123..4197
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label="COX8 presequence"
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
CDS 4228..4245
/label="6xHis"
/note="6xHis affinity tag"
misc_feature 4252..5409
/label="FNR THEEB"
/note="FNR THEEB"
promoter 5443..5772
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 5782..6378
/label="PuroR"
/note="puromycin N-acetyltransferase"
LTR 6674..6907
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 7064..7288
/label="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
regulatory 7331..7340
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
promoter 7384..7488
/label="AmpR promoter"
CDS 7489..8346
/label="AmpR"
/note="beta-lactamase"
rep_origin 8520..9108
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
LTR 9267..9900
/label="3' LTR"
/note="3' long terminal repeat (LTR) from HIV-1"
promoter 10002..11179
/label="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
CDS 11176..11217
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 11314..11431
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
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