pPIPRA587 vector (V004089)

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LOCUS       40924_34684       12429 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPIPRA587, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Bennett AB.
  TITLE     Enabling Technologies for Plant Transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12429)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Plant Sciences, UC Davis, One shields 
            Avenue, Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 12429)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12429)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2011) Plant Sciences, UC Davis, One shields Avenue, Davis, 
            CA 95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..406
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             420..1142
                     /codon_start=1
                     /gene="ipt"
                     /product="isopentenyl phosphotransferase"
                     /label=ipt
                     /note="condensation of AMP and isopentenylpyrophosphate to
                     form isopentenyl-AMP, a cytokinin"
                     /protein_id="AFI23677.1"
                     /translation="MDLHLIFGPTCTGKTTTAIALAQQTGLPVLSLDRVQCCPQLSTGS
                     GRPTVEELKGTTRLYLDDRPLVEGIIAAKQAHHRLIEEVYNHEANGGLILEGGSTSLLN
                     CMARNSYWSADFRWHIIRHKLPDQETFMKAAKARVKQMLHPAAGHSIIQELVYLWNEPR
                     LRPILKEIDGYRYAMLFASQNQITADMLLQLDANMEGKLINGIAQEYFIHARQQEQKFP
                     QVNAAAFDGFEGHPFGMY"
     gene            420..1142
                     /gene="ipt"
                     /label=ipt
     terminator      1505..1757
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    2026..2050
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      2137..3080
                     /label=FMV34S
                     /note="FMV34S"
                     /regulatory_class="promoter"
     CDS             3099..3887
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      3897..4173
                     /label=Mas term 3' UTR
                     /note="Mas term 3' UTR"
                     /regulatory_class="terminator"
     terminator      3908..4160
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     regulatory      4127..5160
                     /label=FMV34S
                     /note="FMV34S"
                     /regulatory_class="promoter"
     misc_feature    5172..5226
                     /label=MCS (polylinker)
                     /note="MCS (polylinker)"
     regulatory      5235..5538
                     /label=Pea E9 3' UTR
                     /note="Pea E9 3' UTR"
                     /regulatory_class="terminator"
     misc_feature    5625..5649
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7113..7739
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8171..9241
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9310..9504
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9978..10118
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10304..10892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11360..12151)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"