pPIPRA561 vector (V004090)

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LOCUS       40924_34679       10636 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pPIPRA561, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Chi-Ham CL, Boettiger S, Figueroa-Balderas R, Bird S, Geoola JN, 
            Zamora P, Alandete-Saez M, Bennett AB.
  TITLE     An intellectual property sharing initiative in agricultural 
            biotechnology: development of broadly accessible technologies for 
            plant transformation
  JOURNAL   Plant Biotechnol. J. 10 (5), 501-510 (2012)
  PUBMED    22221977
REFERENCE   2  (bases 1 to 10636)
  AUTHORS   Chi-Ham CL, Geoola JN, Figueroa-Balderas R, Bird S, Zamora P, 
            Boettiger S, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue,
            Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 10636)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10636)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol. J."; date: "2012"; volume: "10"; issue: "5"; pages: 
            "501-510"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue, Davis, CA
            95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    245..269
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      350..1300
                     /gene="FMV34S"
                     /regulatory_class="promoter"
     CDS             1318..2106
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      2127..2379
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     regulatory      2430..3380
                     /gene="FMV34S"
                     /regulatory_class="promoter"
     misc_feature    3391..3445
                     /label=MCS (polylinker)
                     /note="MCS (polylinker)"
     regulatory      3451..3755
                     /label=pea E9 3' UTR
                     /note="pea E9 3' UTR"
                     /regulatory_class="terminator"
     misc_feature    3844..3868
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5320..5946
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6378..7448
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7517..7711
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8185..8325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8511..9099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9567..10358)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"