pPigBase1.4 vector (V004096)

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LOCUS       40924_34634        4284 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPigBase1.4 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Kojin Y, Mon H, Sakashita K, Tatsuke T, Miyagawa Y, Takahashi M, Lee
            J, Kawaguchi Y, Koga K, Kusakabe T.
  TITLE     Efficient RNA interference induction by long hairpin dsRNAs 
            expressed from chromosomes of the silkworm, Bombyx mori
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Kojin Y, Kusakabe T, Lee J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2006) Contact:Takahiro Kusakabe Kyushu University 
            Graduate School, Bioresource and Bioenviroonmental Sciences; 
            Laboratory of Silkworm Sicences, Hakozaki 6-10-1, Higashi-ku, 
            Fukuoka, Fukuoka 812-8581, Japan
REFERENCE   3  (bases 1 to 4284)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4284)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAR-2006) Contact:Takahiro Kusakabe Kyushu University Graduate 
            School, Bioresource and Bioenviroonmental Sciences; Laboratory of 
            Silkworm Sicences, Hakozaki 6-10-1, Higashi-ku, Fukuoka, Fukuoka 
            812-8581, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..430
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      551..1139
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(1442..1666)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1736..2266)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(2455..2483)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   2868..2880
                     /note="piggyBac transposable element, TR R; terminal repeat
                     of piggyBac"
     repeat_region   2884..2902
                     /label=piggyBac transposable element, IR R
                     /note="piggyBac transposable element, IR R"
     protein_bind    complement(3195..3228)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3239..3248
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    3307..3401
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     repeat_region   3629..3648
                     /label=piggyBac transposable element, IR L
                     /note="piggyBac transposable element, IR L"
     repeat_region   3679..3691
                     /note="piggyBac transposable element, TR L; terminal repeat
                     of piggyBac"
     mobile_element  complement(3937..4102)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"