pPIGA3II vector (V004097)

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LOCUS       40924_34629        7172 bp DNA     circular SYN 18-DEC-2018
DEFINITION  PiggyBac donor vector pPIGA3II DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Mitsutake H, Kobayashi J.
  TITLE     piggyBac-mediated stable transformation of cultured Bombyx mori 
            cells using in vitro synthesized transposase mRNA
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Mitsutake H, Kobayashi J.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2010) Contact:Jun Kobayashi Yamaguchi University, 
            Faculty of Agriculture; Yoshida 1677-1, Yamaguchi, Yamaguchi 
            753-8515, Japan URL 
            :http://www.agr.yamaguchi-u.ac.jp/~member/kobayashi/pe.html
REFERENCE   3  (bases 1 to 7172)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7172)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2010) Contact:Jun Kobayashi Yamaguchi University, Faculty of
            Agriculture"; volume: " Yoshida 1677-1, Yamaguchi, Yamaguchi 
            753-8515, Japan URL :http"; pages: 
            "//www.agr.yamaguchi-u.ac.jp/~member/kobayashi/pe.htm"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   556..591
                     /label=piggyBac 5' inverted terminal repeat
                     /note="piggyBac 5' inverted terminal repeat"
     promoter        1313..1765
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             1864..2460
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     misc_feature    2517..2822
                     /gene="PGK"
                     /label=PGK polyadenylation sequence
                     /note="PGK polyadenylation sequence"
     gene            2517..2822
                     /gene="PGK"
                     /label=PGK
     gene            2840..3495
                     /gene="A3"
                     /label=A3
     regulatory      2840..2966
                     /gene="A3"
                     /label=actin A3
                     /note="actin A3"
                     /regulatory_class="promoter"
     exon            2967..3035
                     /gene="A3"
                     /note="actin A3"
     intron          3036..3495
                     /gene="A3"
                     /note="actin A3"
     CDS             3525..4241
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    4440..4463
                     /note="piggyBac transposase polyadenylation sequence; 
                     transposase"
     repeat_region   4565..4627
                     /label=piggyBac 3' inverted terminal repeat
                     /note="piggyBac 3' inverted terminal repeat"
     primer_bind     complement(4951..4967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4975..4991)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4999..5029)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5044..5065)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5353..5941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6115..6972)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6973..7077)
                     /label=AmpR promoter