pPICZBBAX vector (V004101)

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LOCUS       V004101                 3912 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004101
VERSION     V004101
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3912)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..46
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             62..79
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      159..405
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        420..831
                     /label="TEF1 promoter"
                     /note="promoter for EF-1-alpha"
     promoter        839..886
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             905..1276
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      1345..1592
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(1667..2255)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2337..3275
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             3314..3889
                     /gene="Bax"
                     /label="Apoptosis regulator BAX"
                     /note="Apoptosis regulator BAX from Mus musculus.
                     Accession#: Q07813"