pPICZalphaH6E vector (V004102)

LOCUS       40924_34600        3551 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPICZalphaH6E, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Janakiraman VN, Cabanne C, Dieryck W, Hocquellet A, Joucla G, Le 
            Senechal C, Chaignepain S, Costaglioli P, Santarelli X, Garbay B, 
            Noubhani A.
  TITLE     Production and purification of recombinant human hepcidin-25 with 
            authentic N and C-termini
  JOURNAL   J. Biotechnol. 195, 89-92 (2015)
  PUBMED    25562424
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Dieryck W, Narasimhan Janakiraman V, Cabanne C, Hocquellet A, Joucla
            G, Le Senechal C, Chaignepain S, Costaglioli P, Santarelli X, Garbay
            B, Noubhani A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUN-2014) ENSTBB, Institut Polytechnique de Bordeaux, 
            146 rue Leo Saignat, Bordeaux, gironde 33076, France
REFERENCE   3  (bases 1 to 3551)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3551)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 195, 89-92 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JUN-2014) ENSTBB, Institut Polytechnique de Bordeaux, 146 rue 
            Leo Saignat, Bordeaux, gironde 33076, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /codon_start=1
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     CDS             1238..1255
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             1301..1315
                     /codon_start=1
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
                     /translation="DDDDK"
     misc_feature    1315..1359
                     /label=multiple cloning site
                     /note="multiple cloning site"
     terminator      1375..1621
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1636..2047
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2055..2102
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2121..2492
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      2561..2808
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2883..3471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"