Basic Vector Information
- Vector Name:
- pPh115
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4619 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Krom RJ, Bhargava P, Lobritz MA, Collins JJ.
- Promoter:
- PLtetO-1
pPh115 vector Vector Map
pPh115 vector Sequence
LOCUS 40924_34431 4619 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pPh115, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4619)
AUTHORS Krom RJ, Bhargava P, Lobritz MA, Collins JJ.
TITLE Engineered Phagemids for Nonlytic, Targeted Antibacterial Therapies
JOURNAL Nano Lett. 15 (7), 4808-4813 (2015)
PUBMED 26044909
REFERENCE 2 (bases 1 to 4619)
AUTHORS Krom RJ.
TITLE Direct Submission
JOURNAL Submitted (02-JUN-2015) Molecular Medicine, Boston University, 72
East Concord St, Boston, MA 02118, USA
REFERENCE 3 (bases 1 to 4619)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4619)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nano
Lett."; date: "2015"; volume: "15"; issue: "7"; pages: "4808-4813"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-JUN-2015) Molecular Medicine, Boston University, 72 East Concord
St, Boston, MA 02118, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4619
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 31..104
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
CDS 142..264
/codon_start=1
/product="cecropin PR-39"
/label=cecropin PR-39
/protein_id="AKQ98672.1"
/translation="MRRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP"
CDS 313..435
/codon_start=1
/product="cecropin PR-39"
/label=cecropin PR-39
/protein_id="AKQ98673.1"
/translation="MRRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP"
terminator 456..550
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
regulatory 631..704
/label=PLtet0
/note="PLtet0"
/regulatory_class="promoter"
promoter 631..704
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
protein_bind 631..649
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 656..674
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
RBS 730..738
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 745..804
/codon_start=1
/product="apidaecin"
/label=apidaecin
/protein_id="AKQ98674.1"
/translation="MGNNRPVYIPQPRPPHPRI"
RBS 843..851
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 857..916
/codon_start=1
/product="apidaecin"
/label=apidaecin
/protein_id="AKQ98675.1"
/translation="MGNNRPVYIPQPRPPHPRI"
regulatory 923..1045
/label=T0
/note="T0"
/regulatory_class="terminator"
terminator 937..1031
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
regulatory 1064..1137
/label=PLtet0
/note="PLtet0"
/regulatory_class="promoter"
promoter 1064..1137
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
protein_bind 1064..1082
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1089..1107
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 1178..1480
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
regulatory 1490..1612
/label=T0
/note="T0"
/regulatory_class="terminator"
terminator 1504..1598
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
promoter 1619..1692
/label=PLtetO-1 promoter
/note="modified phage lambda PL promoter with tet operator
sites (Lutz and Bujard, 1997)"
protein_bind 1619..1637
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1644..1662
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
CDS 1733..2035
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
terminator 2059..2153
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
rep_origin 2186..2613
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
terminator 2638..2724
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(2888..3476)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator complement(3564..3658)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(3692..4483)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
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