pPh112 vector (V004115)

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LOCUS       V004115                 4461 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004115
VERSION     V004115
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Krom RJ, Bhargava P, Lobritz MA, Collins JJ.
  TITLE     Engineered Phagemids for Nonlytic, Targeted Antibacterial Therapies
  JOURNAL   Nano Lett. 15 (7), 4808-4813 (2015)
   PUBMED   26044909
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Krom RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) Molecular Medicine, Boston University, 72
            East Concord St, Boston, MA 02118, USA
REFERENCE   3  (bases 1 to 4461)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4461)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nano
            Lett."; date: "2015"; volume: "15"; issue: "7"; pages: "4808-4813"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-JUN-2015) Molecular Medicine, Boston University, 72 East Concord
            St, Boston, MA 02118, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..104
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     CDS             142..264
                     /codon_start=1
                     /product="cecropin PR-39"
                     /label="cecropin PR-39"
                     /protein_id="AKQ98660.1"
                     /translation="MRRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP"
     CDS             313..435
                     /codon_start=1
                     /product="cecropin PR-39"
                     /label="cecropin PR-39"
                     /protein_id="AKQ98661.1"
                     /translation="MRRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP"
     terminator      456..550
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     regulatory      631..704
                     /label="PLtet0"
                     /note="PLtet0"
                     /regulatory_class="promoter"
     promoter        631..704
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     protein_bind    631..649
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    656..674
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             730..738
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             745..804
                     /codon_start=1
                     /product="apidaecin"
                     /label="apidaecin"
                     /protein_id="AKQ98662.1"
                     /translation="MGNNRPVYIPQPRPPHPRI"
     RBS             843..851
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             857..916
                     /codon_start=1
                     /product="apidaecin"
                     /label="apidaecin"
                     /protein_id="AKQ98663.1"
                     /translation="MGNNRPVYIPQPRPPHPRI"
     regulatory      923..1045
                     /label="T0"
                     /note="T0"
                     /regulatory_class="terminator"
     terminator      937..1031
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     regulatory      1064..1137
                     /label="PLtet0"
                     /note="PLtet0"
                     /regulatory_class="promoter"
     promoter        1064..1137
                     /label="PLtetO-1 promoter"
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1064..1082
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1089..1107
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1170..1478
                     /gene="parE"
                     /label="Toxin ParE"
                     /note="Toxin ParE from Escherichia coli. Accession#:
                     Q79EC5"
     CDS             1533..1841
                     /gene="parE"
                     /label="Toxin ParE"
                     /note="Toxin ParE from Escherichia coli. Accession#:
                     Q79EC5"
     regulatory      1851..1973
                     /label="T0"
                     /note="T0"
                     /regulatory_class="terminator"
     terminator      1865..1959
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     rep_origin      2028..2455
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      2480..2566
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2730..3318)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(3406..3500)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3534..4325)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"