pFS186-PCa2-LuxS vector (V006186)

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LOCUS       V006186                 4205 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006186
VERSION     V006186
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Designer cells programming quorum-sensing interference with human
            pathogens
  JOURNAL   Nat Commun (2018) In press
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Sedlmayer F, Hell D, Mueller M, Auslaender D, Fussenegger M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-2017) Department of Biosystems Science and
            Engineering (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058,
            Switzerland
REFERENCE   3  (bases 1 to 4205)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4205)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun
            (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-AUG-2017) Department of Biosystems Science and Engineering
            (D-BSSE), ETH Zurich, Mattenstrasse 26, Baseel 4058, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             9..521
                     /gene="luxS"
                     /label="S-ribosylhomocysteine lyase"
                     /note="S-ribosylhomocysteine lyase from Escherichia coli
                     (strain K12). Accession#: P45578"
     polyA_signal    complement(563..684)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     regulatory      787..1032
                     /label="simian virus 40 polyA"
                     /note="simian virus 40 polyA"
                     /regulatory_class="polyA_signal_sequence"
     rep_origin      complement(1349..1937)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2111..2968)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2969..3073)
                     /label="AmpR promoter"
     rep_origin      3100..3555
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    3686..3734
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    3748..3839
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    4071..4100
                     /label="NFAT binding site"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    4101..4130
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    4131..4160
                     /label="NFAT binding site"
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     promoter        4173..4204
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"