pFS-sigHis-PGK vector (V006188)

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LOCUS       40924_20541        3084 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pFS-sigHis-PGK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Wilke S, Gossen M, Wirth D, Buessow K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2011) Structural Biology, Helmholtz Centre for 
            Infection Research, Inhoffenstr. 7, Braunschweig 38124, Germany
REFERENCE   2  (bases 1 to 3084)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3084)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (09-FEB-2011) Structural Biology, Helmholtz Centre for Infection 
            Research, Inhoffenstr. 7, Braunschweig 38124, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     25..72
                     /note="F3 site; synthetic variant 3 of the yeast
                     flp-recombinase target (FRT) site"
     CDS             91..147
                     /label=IgH signal sequence
                     /note="signal sequence from the mouse immunoglobulin heavy
                     chain variable region"
     misc_feature    148..175
                     /label=BpiI cloning site
                     /note="BpiI cloning site"
     CDS             176..193
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    complement(211..345)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        683..1193
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     protein_bind    1220..1267
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      complement(1332..1920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2094..2951)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2952..3056)
                     /label=AmpR promoter
     primer_bind     3067..3084
                     /label=pBR1
                     /note="pBR1"