pFRT2-lux-Tel vector (V006189)

LOCUS       40924_20536       11266 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fusion vector pFRT2-lux-Tel, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Wilcox BA, Hoang TT.
  TITLE     Engineering of tellurite-resistant genetic tools for single-copy 
            chromosomal analysis of Burkholderia spp. and characterization of 
            the Burkholderia thailandensis betBA operon
  JOURNAL   Appl. Environ. Microbiol. 75 (12), 4015-4027 (2009)
  PUBMED    19376905
REFERENCE   2  (bases 1 to 11266)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Videau PJA., Wilcox BA, Hoang TT.
  TITLE     Genetic Systems for Single Copy Analysis based on a Non-antibiotic 
            Selectable Marker in Burkholderia spp.
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 11266)
  AUTHORS   Kang Y, Norris MH, Barrett AR, Videau PJA., Wilcox BA, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2008) Microbiology, University of Hawaii at Manoa,
            2538 McCarthy Mall, Snyder Hall 207, Honolulu, HI 96822, USA
REFERENCE   4  (bases 1 to 11266)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 11266)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2009"; volume: "75"; issue: "12"; 
            pages: "4015-4027"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2008) Microbiology, University of Hawaii at Manoa, 2538 
            McCarthy Mall, Snyder Hall 207, Honolulu, HI 96822, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(190..299)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     terminator      complement(720..747)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(839..925)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(1549..2658)
                     /label=LuxE
                     /note="LuxE"
     CDS             complement(2840..3820)
                     /label=LuxB
                     /note="LuxB luciferase subunit"
     CDS             complement(3838..4917)
                     /label=LuxA
                     /note="LuxA luciferase subunit"
     CDS             complement(4969..5889)
                     /label=LuxD
                     /note="LuxD acyltransferase"
     CDS             complement(5904..7343)
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
     protein_bind    complement(7408..7455)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7558..7586
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     terminator      7710..7741
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(7759..8712)
                     /codon_start=1
                     /gene="telB"
                     /product="TelB"
                     /label=telB
                     /note="tellurite resistance"
                     /protein_id="ACJ70732.1"
                     /translation="MNATNTDVFAQVGGLEARGAKMKKRGTRFLIAALAVLAIAGIGAV
                     TGWAISPSATPGSIDVPQVLASTFSDQVPGSEGGGLGGGLPFTSAVGAFTDFMAGPAIF
                     TLGILGIVVAGAVLVFGGEFCGFVRSVCMMVIAVSMIFVSSNLVKGILGGDHDAGPAEP
                     SPRARFMAAVEAKDFARVQELIEARGAKSAADYVLAQLAVAEGLDRKPGARVVVGKAAG
                     SMAMPPAALGFTPRGEAAYAIERSAYGEPRSSIAKQYQQEWNRKAATWWAMAGVAGIIG
                     AILAAAATGFVGLAVSIRNRVKRVRDLLVMEPGAEP"
     gene            complement(7759..8712)
                     /gene="telB"
                     /label=telB
     CDS             complement(8709..9845)
                     /codon_start=1
                     /gene="telA"
                     /product="TelA"
                     /label=telA
                     /note="tellurite resistance"
                     /protein_id="ACJ70733.1"
                     /translation="MNALKTTHDAKAPIVAFDMTPATLRELGLQESDVPEVHAVAQRIE
                     VGSPQTVAEFGRDVAEHTSRYADSLLDQVRNSDLDEAGEKLTQVVAKARSLNVGPLSDN
                     RSRLPLIGPLIDRFRVRSTGFMARFDTTREQIEHLVSEVQTTQQGIAQRNASLDEMFAA
                     VREEHRLLGVHIAAGKVRLAELREQAEGLRGNVGNDPGRVQELADLDAMVANLDKRIGD
                     LIALQHSAMQSLPTIRMIQANNQMLVDKFHTIREITVPAWKRQFMLALSLNEQKNAVEL
                     ATAIDDTTNDLMKRNAALLHRTSVETAKENQRLVIDVDTLKQVQTTLIKTVEDVIRIQQ
                     EGVQKRKDAEKQIAAMRGDLQAKLTRQPVRELAQQESV"
     gene            complement(8709..9845)
                     /gene="telA"
                     /label=telA
     CDS             complement(9863..10636)
                     /codon_start=1
                     /gene="kilA"
                     /product="KilA"
                     /label=kilA
                     /note="tellurite resistance"
                     /protein_id="ACJ70734.1"
                     /translation="MEEQSVNMARLKGEVLPALFASPATIGEYGAGIDGADSLNELSNL
                     MEHGAVAALADKISQIVAKLADADPRKIAEKPTWFEKMLGREVERQVRYQVARKTLDQL
                     LDEAEGVAQRVRDTLRALDDMLNTHEAEVDRLRAYIQAGREFLDENPEAGAAKAGVIEF
                     DKPRERFARKLANLATLMASHEMSVTQMKLTRAQAVDMLDRFSETASVLVPVWRQHTLA
                     LITTKNMNPAMVAEAAKAHQALMRSLSQSLEGINQ"
     gene            complement(9863..10636)
                     /gene="kilA"
                     /label=kilA
     RBS             complement(10643..10665)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      10676..10717
                     /note="derived from Burkholderia cenocepacia rpsL promoter"
                     /regulatory_class="promoter"
     rep_origin      10857..11242
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"