pFRK1-LUC-NOS-At2g19190 vector (V006196)

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LOCUS       40924_20496        7525 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Control vector pFRK1-LUC-NOS-At2g19190, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, 
            Boller T, Ausubel FM, Sheen J.
  TITLE     MAP kinase signalling cascade in Arabidopsis innate immunity
  JOURNAL   Nature 415 (6875), 977-983 (2002)
  PUBMED    11875555
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   He P, Shan L, Lin NC, Martin GB, Kemmerling B, Nurnberger T, Sheen 
            J.
  TITLE     Specific bacterial suppressors of MAMP signaling upstream of MAPKKK 
            in Arabidopsis innate immunity
  JOURNAL   Cell 125 (3), 563-575 (2006)
  PUBMED    16678099
REFERENCE   3  (bases 1 to 7525)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   4  (bases 1 to 7525)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7525)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2002"; volume: "415"; issue: "6875"; pages: "977-983"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Cell"; 
            date: "2006"; volume: "125"; issue: "3"; pages: "563-575"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2141)
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2667..5461
                     /label=FRK1 promoter
                     /note="FRK1 promoter"
                     /regulatory_class="promoter"
     CDS             5462..7111
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      7269..7520
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"