pFNK-202-qsc119 vector (V006209)

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LOCUS       V006209                 3866 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006209
VERSION     V006209
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Karig D, Martini KM, Lu T, DeLateur NA, Goldenfeld N, Weiss R.
  TITLE     Stochastic Turing patterns in a synthetic bacterial population
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2018) In press
   PUBMED   29891706
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Karig DK.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Research and Development Department, Johns
            Hopkins University Applied Physics Laboratory, 11100 Johns Hopkins
            Rd, Laurel, MD 20723, USA
REFERENCE   3  (bases 1 to 3866)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3866)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-MAY-2018) Research and Development Department, Johns Hopkins
            University Applied Physics Laboratory, 11100 Johns Hopkins Rd,
            Laurel, MD 20723, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(138..860)
                     /gene="rhlR"
                     /label="HTH-type quorum-sensing regulator RhlR"
                     /note="HTH-type quorum-sensing regulator RhlR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P54292"
     RBS             867..878
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      complement(870..878)
                     /label="RBSII"
                     /note="RBSII"
                     /regulatory_class="ribosome_binding_site"
     regulatory      complement(884..939)
                     /regulatory_class="promoter"
     regulatory      complement(893..898)
                     /regulatory_class="minus_10_signal"
     regulatory      complement(916..921)
                     /regulatory_class="minus_35_signal"
     misc_feature    1018..1037
                     /label="qsc119 rhl box"
                     /note="qsc119 rhl box"
     RBS             1092..1103
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      1092..1100
                     /label="RBSII"
                     /note="RBSII"
                     /regulatory_class="ribosome_binding_site"
     CDS             1110..1823
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      1932..2018
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2188..2733)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(2847..2941)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2975..3766)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"