pFN31A vector (V006211)

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LOCUS       40924_20396        6308 bp DNA     circular SYN 18-DEC-2018
DEFINITION  NanoLuc CMV-Hygro luciferase vector pFN31A, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   McNamara B, Kopish K, Robers M.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2013) Scientific Communications, Promega 
            Corporation, 5500 E. Cheryl Parkway, Madison, WI 53711, USA
REFERENCE   2  (bases 1 to 6308)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6308)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (31-OCT-2013) Scientific Communications, Promega Corporation, 5500 
            E. Cheryl Parkway, Madison, WI 53711, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1065..1577
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     misc_feature    1578..1589
                     /gene="NLuc"
                     /label=linker
                     /note="linker"
     misc_feature    1590..1597
                     /gene="NLuc"
                     /note="used in Flexi vector cloning; SgfI site"
     CDS             1621..1953
                     /label=barnase
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     misc_feature    1958..1965
                     /label=PmeI site
                     /note="PmeI site"
     misc_feature    1966..2021
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     polyA_signal    complement(2126..2247)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2492..2849
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2880..3914
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3941..3989
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        4145..4249
                     /label=AmpR promoter
     CDS             4250..5107
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(5268..5856)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5972..6255
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"