pFMIRE6 vector (V006215)

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LOCUS       V006215                12044 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006215
VERSION     V006215
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Differential cleavage rates of concatenated identical miRNA/siRNA
            target sites indicate RISC approaching its target in 5' to 3'
            direction
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   Zhang C, Chen ZJ.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 12044)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12044)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-FEB-2013) Section of Molecular Cell "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label="MAS terminator"
                     /note="mannopine synthase terminator"
     CDS             complement(376..924)
                     /label="BlpR"
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(930..1310)
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        1410..2081
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    2227..2256
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     misc_feature    2257..2468
                     /label="contains 35S polyA signal"
                     /note="contains 35S polyA signal"
     promoter        2890..3234
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3378..3812
                     /note="Histone H2B.10 from Arabidopsis thaliana.
                     Accession#: Q9FFC0"
                     /label="Histone H2B.10"
     CDS             3831..4538
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     polyA_signal    4566..4740
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     terminator      4812..5519
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     primer_bind     complement(5548..5564)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    5767..5791
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7091..7717
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8154..9218
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9287..9481
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9825..9965
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10151..10739)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10829..11620)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"