pFHC2101 vector (V006253)

LOCUS       40924_20061        4694 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pFHC2101, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Atlung T, Hansen FG.
  TITLE     Effect of different concentrations of H-NS protein on chromosome 
            replication and the cell cycle in Escherichia coli
  JOURNAL   J. Bacteriol. 184 (7), 1843-1850 (2002)
  PUBMED    11889089
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Hansen FG, Atlung T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2001) BioCentrum-DTU, Technical University of 
            Denmark, Building 301, Lyngby DK-2800, Denmark
REFERENCE   3  (bases 1 to 4694)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4694)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Bacteriol."; date: "2002"; volume: "184"; issue: "7"; pages: 
            "1843-1850"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2001) BioCentrum-DTU, Technical University of Denmark, 
            Building 301, Lyngby DK-2800, Denmark"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4694
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1
                     /label=SP6 transcription initiation site
                     /note="SP6 transcription initiation site"
     misc_feature    10..74
                     /note="multiple cloning site; synthetic adapters"
     regulatory      58..64
                     /gene="lacI"
                     /label=Shine-Dalgarno sequence
                     /note="Shine-Dalgarno sequence"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    89..107
                     /gene="lacI"
                     /label=lac operator (O)
                     /note="lac operator (O)"
     protein_bind    91..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(123..139)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        383..460
                     /label=lacI promoter
     CDS             461..1540
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1850..1858
                     /label=DnaA binding site
                     /bound_moiety="DnaA"
     rep_origin      complement(1945..2533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2707..3564)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3565..3669)
                     /label=AmpR promoter
     misc_feature    3949..3950
                     /note="inserted with Klenow polymerase to remove NdeI site"
     rep_origin      4005..4460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(4559..4606)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        4678..4694
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"