pFGPDAngls2a vector (V006262)

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LOCUS       40924_20021        8719 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Fungal expression vector pFGPDAngls2a, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8719)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8719)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(403..947)
                     /label=trpC terminator
                     /note="trpC terminator"
                     /regulatory_class="terminator"
     misc_feature    complement(1025..1105)
                     /label=3' UTR of A. niger gls2a
                     /note="3' UTR of A. niger gls2a"
     exon            complement(1106..3170)
                     /note="exon 3 of A. niger gls2a"
     intron          complement(3171..3224)
                     /note="intron 2 of A. niger gls2a"
     exon            complement(3225..3966)
                     /note="exon 2 of A. niger gls2a"
     intron          complement(3967..4030)
                     /note="intron 1 of A. niger gls2a"
     exon            complement(4031..4097)
                     /note="exon 1 of A. niger gls2a"
     misc_feature    complement(4098..4183)
                     /label=5' UTR of A. niger gls2a
                     /note="5' UTR of A. niger gls2a"
     intron          complement(4196..4311)
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     promoter        complement(4360..6488)
                     /label=gpdA promoter
                     /note="promoter from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     primer_bind     complement(6498..6514)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6522..6538)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6546..6576)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6591..6612)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6900..7488)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7662..8519)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8520..8624)
                     /label=AmpR promoter